| Объем | 50 мкг |
| Синонимы | Putative POU domain, class 5, transcription factor 1B (Oct4-pg1) (Octamer-binding protein 3-like) (Octamer-binding transcription factor 3-like), POU5F1B, OCT4PG1 OTF3C OTF3P1 POU5F1P1 POU5FLC20 POU5FLC8 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q06416 |
| Иммуноген | Recombinant Human Putative POU domain, class 5, transcription factor 1B protein (10-138AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500 |
| Примечание | Shows weak transcriptional activator activity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Putative POU domain, class 5, transcription factor 1B |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate
All lanes: POU5F1B antibody at 6.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
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IHC image of CSB-PA23129A0Rb diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA23129A0Rb diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: POU5F1B Antibody.