Phospho-STAT3 (S727) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3 | | Тип антител | Recombinant Antibody | | Species | Human | | UniProt ID | P40763 | | Иммуноген | A synthesized peptide derived from human Phospho-STAT3 (S727) | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 | | Клональность | Monoclonal | | Изотип | Rabbit IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Affinity-chromatography | | Области исследований | Signal Transduction | | Аббревиатура | Signal transducer and activator of transcription 3 | | Примечание | Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in:A549 whole cell lysate(treated with EGF or not)
All lanes:Phospho-STAT3 antibody at 1.88µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 98 KDa
Observed band size: 98 KDa
 | IHC image of CSB-RA022812A727phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunoprecipitating Phospho-STAT3 in 293 whole cell lysate treated with Calyculin A
Lane 1: Rabbit control IgG(1µg)instead of CSB-RA022812A727phHU in 293 whole cell lysate treated with Calyculin A.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA022812A727phHU(3µg)+ 293 whole cell lysate treated with Calyculin A(1mg)
Lane 3: 293 whole cell lysate treated with Calyculin A (20µg)
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-RA022812A727phHU | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-STAT3 (S727) Antibody.
Примечание: дополнительная информация (на английском языке). |
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