Phospho-PRKACA (Thr197) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | PRKACA , PRKACB, PKACA, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P17612 | | Иммуноген | Peptide sequence around phosphorylation site of threonine 197(R-T-W(p)-T-L) derived from Human PKA alpha/beta CAT. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 | | Примечание | <p>Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. <br/><br/>Ghislaine Garrel, Endocrinology, Jun 1997, 138: 2259. <br />W Li, Learn. Mem., Jan 1996, 2: 320 - 333. <br />Sharmin Schauble, J. Biol. Chem., May 2007, 282: 14952 - 14959. <br />Yong G. Wang, J. Gen. Physiol., Jan 1998, 111: 113.<br /></p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Thr197 | | Области исследований | Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from mouse brain cells using PKA ?/? CAT (Phospho-Thr197) Antibody.The lane on the right is treated with the antigen-specific peptide.
 | Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA ?/? CAT (Phospho-Thr197) antibody (left)or the same antibody preincubated with blocking peptide (right).
 | Immunofluorescence staining of methanol-fixed A549 cells using PKA ?/? CAT (Phospho-Thr197) Antibody.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA157769 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-PRKACA (Thr197) Antibody.
Примечание: дополнительная информация (на английском языке). |
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