Phospho-NFKB1 (Ser337) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | p50, KBF1, NF-kB1, NFKB-p50, NFkappaB | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P19838 | | Иммуноген | Peptide sequence around phosphorylation site of serine 337(R-K-S(p)-D-L) derived from Human NFκB-p105/p50. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 | | Примечание | <p>NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling, active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.</p><p></p><p>Beg A.A., Baldwin A.S. Jr.Oncogene 9:1487-1492(1994)</p><p>Guizani-Tabbane L., Ben-Aissa K., Belghith M., Sassi A., Dellagi K.Infect. Immun. 72:2582-2589(2004)</p><p>Beinke S., Robinson M.J., Hugunin M., Ley S.C.Mol. Cell. Biol. 24:9658-9667(2004)</p><p></p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Ser337 | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology,Immunology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappa;B p105/p50 (phospho-Ser337) antibody.
 | Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-kappa;B p105/p50 (phospho-Ser337) antibody.
 | Western blot analysis of extract from HeLa cells, using NF-kappa;B p105/p50 (phospho-Ser337) antibody (Lane 1 and 2).
 | Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using NF-kappa;B-p105/p50(Phospho-Ser337) Antibody.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA632717 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-NFKB1 (Ser337) Antibody.
Примечание: дополнительная информация (на английском языке). |
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