| Объем | 100 мкл |
| Синонимы | double minute 4 protein, Mdm2-like p53-binding protein, MDMX, p53-binding protein Mdm4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O15151 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 367 (T-I-S(p)-A-P) derived from Human MDM4. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 |
| Примечание | Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser367 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology,Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MDM4 (Phospho-Ser367) antibody. The lane on the right is treated with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MDM4 (Phospho-Ser367) antibody. The picture on the right is treated with the synthesized peptide.
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Наименование: Phospho-MDM4 (Ser367) Antibody.