| Объем | 100 мкл |
| Синонимы | G3BP, GAP SH3-domain binding protein 1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13283 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.<br/><br/>Tao WA, et al. (2005) Nat Methods. 2(8): 591-598.<br/>Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.<br/>Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135.<br/>Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser232 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells untreated or treated with starvation using G3BP-1(Phospho-Ser232) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1(Phospho-Ser232) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using G3BP-1(Phospho-Ser232) Antibody.
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Наименование: Phospho-G3BP1 (Ser232) Antibody.