| Объем | 100 мкл |
| Синонимы | PYR1, CAD protein, EC 2.1.3.2, EC 3.5.2.3, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P27708 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 456 (P-I-T(p)-P-H) derived from Human CAD. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, IHC,IHC:1:50-1:100 |
| Примечание | <p>The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.<br/><br/>Iwahana H., Biochem. Biophys. Res. Commun. 219:249-255(1996).<br />Davidson J.N., DNA Cell Biol. 9:667-676(1990).<br />Olsen J.V., Cell 127:635-648(2006).<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr456 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology,Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Наименование: Phospho-CAD (Thr456) Antibody.