| Объем | 50 мкг |
| Синонимы | Negative elongation factor B (NELF-B) (Cofactor of BRCA1), NELFB, COBRA1 KIAA1182 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q8WX92 |
| Иммуноген | Recombinant Human Negative elongation factor B protein (8-199AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, ChIP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Negative elongation factor B antibody at 2µg/ml
Lane 1: EC109whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
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Western Blot
All lanes: Negative elongation factor B antibody at 2µg/ml
Lane 1: NIH/3T3 whole cell lysate
Lane 2: HL60 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
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Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA03765A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA03765A0Rb at dilution of 1:100
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Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-COBRA1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP03765HU) against the TFF1 promoter.
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Наименование: NELFB Antibody.