| Объем | 100 мкл |
| Синонимы | Eyes absent homolog 3 (EC 3.1.3.48), EYA3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q99504 |
| Иммуноген | Recombinant Human Eyes absent homolog 3 protein (1-300AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 (PubMed:19234442, PubMed:19351884). Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Eyes absent homolog 3 |
| Области исследований | Epigenetics and Nuclear Signaling, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: EYA3 antibody at 1.49µg/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: HT29 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 63, 49, 59, 57, 58 kDa
Observed band size: 63 kDa
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Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA860766DSR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA860766DSR1HU at dilution of 1:100
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Наименование: EYA3 Antibody.