| Объем | 50 мкл |
| Синонимы | Eukaryotic translation initiation factor 3 subunit K (eIF3k) (Eukaryotic translation initiation factor 3 subunit 12) (Muscle-specific gene M9 protein) (PLAC-24) (eIF-3 p25) (eIF-3 p28), EIF3K, EIF3S12 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UBQ5 |
| Иммуноген | Recombinant Human Eukaryotic translation initiation factor 3 subunit K protein (1-218AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Eukaryotic translation initiation factor 3 subunit K |
| Области исследований | Epigenetics and Nuclear Signaling, Neuroscience |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: EIF3K antibody at 1.72µg/ml
Lane 1: THP-1 whole cell lysate
Lane 2: HT29 whole cell lysate
Lane 3: 293T whole cell lysate
Lane 4: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26, 25 kDa
Observed band size: 26 kDa
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA866204ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA866204ESR1HU at dilution of 1:100
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Immunoprecipitating EIF3K in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA866204ESR1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA866204ESR1HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: EIF3K Antibody.