ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

MBP Antibody

Спецификация

Объем50 мкг
СинонимыMyelin basic protein (MBP) (Myelin A1 protein) (Myelin membrane encephalitogenic protein), MBP
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotP02686
ИммуногенRecombinant Human Myelin basic protein (1-197AA)
ИсточникRabbit
Видовая специфичностьHuman, Mouse, Rat
ПрименениеELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500
ПримечаниеThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки>95%, Protein G purified
АбревеатураMyelin basic protein
Области исследованийNeuroscience, Tags & Cell Markers
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: Rat brain tissue, Rat liver tissue, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue, Mouse kidney tissue
All lanes: MBP antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 22, 21, 19, 18 kDa
Observed band size: 34 kDa

IHC image of CSB-PA013551LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA013551LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of Hela cells using CSB-PA013551LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкг 
Кат. номер:CSB-PA013551LA01HU
Цена (с НДС 20%):по запросуВ корзину
MBP AntibodyНаименование: MBP Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43