| Объем | 100 мкг |
| Синонимы | Heat shock protein HSP 90-beta (HSP 90) (Heat shock 84 kDa) (HSP 84) (HSP84), HSP90AB1, HSP90B HSPC2 HSPCB |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P08238 |
| Иммуноген | Recombinant Human human Heat shock protein HSP 90-beta protein (150-355AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat, Zebrafish |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
| Примечание | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Hsp90ab1 antibody at 2µg/ml
Lane 1: Mouse lung tissue
Lane 2: Rat lung tissue
Lane 3: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 83, 120, 32 kDa
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Western Blot
Positive WB detected in: HepG2 cell
All lanes: CHD4 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
 |
Western Blot
Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: HSP90AB1 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
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IHC image of CSB-PA00109A0Rb diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of MCF-7 cells with CSB-PA00109A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: HSP90AB1 Antibody.