HMGB4 AntibodyСпецификация| Объем | 50 мкл | | Синонимы | high mobility group box 4 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q8WW32 | | Иммуноген | Fusion protein of human HMGB4 | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB,ELISA:1:1000-1:2000, WB:1:200-1:1000 | | Примечание | High mobility group protein B4 is a transcription factor that in humans is encoded by the HMGB4 gene.HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues. HMGB4 associates with chromatin, and in transfection assays, in contrast to HMGB1, it acts as a potent transcriptional repressor. During spermatogenesis, HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids, whereas stronger expression is observed during the elongation phase, where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole. HMGB4 basal localization is lost in H1FNT-mutant spermatids, showing that H1FNT provides a positional cue for organizing chromatin domains within the nucleus. These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen affinity purification | | Области исследований | Others | | Ссылка на страницу на сайте производителя | ссылка | Gel: 12%SDS-PAGE, Lysate: 40 ?g, Lane 1-2: 231 cells, human fetal liver tissue, Primary antibody: CSB-PA049190(HMGB4 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
 | | | |
Информация для заказа |
Наименование: HMGB4 Antibody.