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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

HIST1H4A (Ab-12) Antibody

Спецификация

Объем100 мкл
СинонимыHistone H4, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4, H4/A H4FA, H4/I H4FI, H4/G H4FG, H4/B H4FB, H4/J H4FJ, H4/C H4FC, H4/H H4FH, H4/M H4FM, H4/E H4FE, H4/D H4FD, H4/K H4FK, H4/N H4F2 H4FN HIST2H4, H4/O H4FO,
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotP62805
ИммуногенPeptide sequence around site of butyrly-Lys (12) derived from Human Histone H4
ИсточникRabbit
Видовая специфичностьHuman
ПрименениеELISA, WB, IHC, IP, ChIP, Recommended dilution: WB:1:200-1:2000, IHC:1:10-1:100, IP:1:200-1:2000
ПримечаниеCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очисткиAntigen Affinity Purified
АбревеатураHistone H4
Области исследованийEpigenetics and Nuclear Signaling
Ссылка на страницу на сайте производителяссылка
IHC image of CSB-PA010429OA12nbutHU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA010429OA12nbutHU diluted at 1:100 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (CSB-PA010429OA12nbutHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: HIST1H4A antibody at 0.16µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa

Immunoprecipitating HIST1H4A in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010429OA12nbutHU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010429OA12nbutHU (3µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)

  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:100 мкл 
Кат. номер:CSB-PA010429OA12nbutHU
Цена (с НДС 20%):по запросуВ корзину
HIST1H4A (Ab-12) AntibodyНаименование: HIST1H4A (Ab-12) Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43