| Объем | 100 мкг |
| Синонимы | Histone deacetylase 3 (HD3) (EC 3.5.1.98) (RPD3-2) (SMAP45), HDAC3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O15379 |
| Иммуноген | Recombinant Human Histone deacetylase 3 protein (1-428AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, ChIP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1, increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Histone deacetylase 3 antibody at 2µg/ml + Hela cells
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49, 50 kDa
Observed band size: 49 kDa
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Western Blot
Positive WB detected in: Mouse heart
All lanes: HDAC3 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 50 kDa
Observed band size: 49 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA010239LA01HU at dilution of 1:100
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Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HDAC3 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP010239HU) against the P21 promoter.
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Immunofluorescent analysis of Hela cells using CSB-PA010239LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: HDAC3 Antibody.