| Объем | 50 мкл |
| Синонимы | Guanylate-binding protein 1 (EC 3.6.5.-) (GTP-binding protein 1) (GBP-1) (HuGBP-1) (Guanine nucleotide-binding protein 1) (Interferon-induced guanylate-binding protein 1), GBP1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P32455 |
| Иммуноген | Recombinant Human Interferon-induced guanylate-binding protein 1 protein (383-592AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Hydrolyzes GTP to GMP in two consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Immunology, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Interferon-induced guanylate-binding protein 1 antibody at 4µg/ml
Lane 1: MM231 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: MGF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA009297ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA009297ESR2HU at dilution of 1:100
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Immunoprecipitating GBP1 in MCF-7 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA009297ESR2HU in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA009297ESR2HU (4µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (20µg)
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Наименование: GBP1 Antibody.