GABBR1 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | GABABR2, GPR51, G protein-coupled receptor 51, GABA-B RECEPTOR, R2 SUBUNIT | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q9UBS5 | | Иммуноген | Synthesized peptide derived from human GABA B receptor. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 | | Примечание | Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen. Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.<br/><br/>Xinmin Xie, J. Physiol., Jul 2006, 574: 399 - 414.<br/>A. Gambardella, Neurology, Feb 2003, 60: 560 - 563.<br/>P Tosetti, J. Physiol., Nov 2005, 568: 951 - 966.<br/>Marta Margeta-Mitrovic, PNAS, Dec 2001, 98: 14643.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Neuroscience | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human brain tissue using GABA B Receptor antibody.
 | Western blot analysis of extracts from HeLa cells, using GABA B Receptor antibody.
 | Western blot analysis of extracts from JK cells (Lane 2) and A549 cells (Lane 3), using GABA-B Receptor antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: GABBR1 Antibody.