| Объем | 100 мкл |
| Синонимы | G3BP, GAP SH3-domain binding protein 1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13283 |
| Иммуноген | Peptide sequence around aa.230~234 (S-S-S-P-A) derived from Human G3BP-1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 |
| Примечание | May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.<br/><br/>Tao WA, et al. (2005) Nat Methods. 2 (8): 591-598.<br/>Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.<br/>Beausoleil SA, et al. (2004) Proc Natl Acad Sci USA.101 (33): 12130-12135.<br/>Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells using G3BP-1(Ab-232) Antibody and the same antibody preincubated with blocking peptide.
 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1(Ab-232) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 |
| | |
Наименование: G3BP1 (Ab-232) Antibody.