| Объем | 100 мкг |
| Синонимы | Eukaryotic translation initiation factor 3 subunit A (eIF3a) (Eukaryotic translation initiation factor 3 subunit 10) (eIF-3-theta) (eIF3 p167) (eIF3 p180) (eIF3 p185), EIF3A, EIF3S10 KIAA0139 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14152 |
| Иммуноген | Recombinant Human Eukaryotic translation initiation factor 3 subunit A protein (1288-1379AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
| Примечание | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Eukaryotic translation initiation factor 3 subunit A |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: EIF3A antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 167, 163 kDa
Observed band size: 167 kDa
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Immunofluorescent analysis of PC-3 cells using CSB-PA623903LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: EIF3A Antibody.