CHEK2 AntibodyСпецификация| Объем | 50 мкл | | Синонимы | Checkpoint kinase 2 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | O96017 | | Иммуноген | Synthetic peptide of human CHEK2 | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB,ELISA:1:1000-1:2000, WB:1:200-1:1000 | | Примечание | In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen affinity purification | | Области исследований | Epigenetics and Nuclear Signaling,Cancer | | Ссылка на страницу на сайте производителя | ссылка | Gel: 6%SDS-PAGE, Lysate: 40 ?g, Lane 1-5: MCF7 cells, 293T cells, HT29 cells, Hela cells, Jurkat cells, Primary antibody: CSB-PA187496(CHEK2 Antibody) at dilution 1/150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
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Наименование: CHEK2 Antibody.