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/ Каталог / Реагенты для научных исследований / Антитела / Рекомбинантные антитела Cusabio

CDC5L Antibody

Спецификация

Объем100 мкл
СинонимыCell division cycle 5-like protein, Cdc5-like protein, Pombe cdc5-related protein, CDC5L, KIAA0432, PCDC5RP
Тип антителRecombinant Antibody
SpeciesHuman
UniProt IDQ99459
ИммуногенA synthesized peptide derived from human CDC5L
Видовая специфичностьHuman
ПрименениеELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
КлональностьMonoclonal
ИзотипRabbit IgG
КоньюгатNon-conjugated
БуфферRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очисткиAffinity-chromatography
Области исследованийEpigenetics and Nuclear Signaling
АббревиатураCell division cycle 5-like protein
ПримечаниеDNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
All lanes: CDC5L antibody at 1.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 93 KDa
Observed band size: 100 KDa

IHC image of CSB-RA858712A0HU diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA858712A0HU diluted at 1:126.5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CSB-RA858712A0HU at 1:42, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating CDC5L in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA858712A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA858712A0HU (3µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)

  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:100 мкл 
Кат. номер:CSB-RA858712A0HU
Цена (с НДС 20%):по запросуВ корзину
CDC5L AntibodyНаименование: CDC5L Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43