| Объем | 100 мкг |
| Синонимы | Mitotic checkpoint protein BUB3, BUB3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O43684 |
| Иммуноген | Recombinant Human Mitotic checkpoint protein BUB3 protein (6-217AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200 |
| Примечание | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Mitotic checkpoint protein BUB3 |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue
All lanes: BUB3 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38, 37 kDa
Observed band size: 38, 37 kDa
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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA002884LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA002884LA01HU at dilution of 1:100
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Наименование: BUB3 Antibody.