| Объем | 50 мкг |
| Синонимы | Serine/threonine-protein kinase WNK3 (EC 2.7.11.1) (Protein kinase lysine-deficient 3) (Protein kinase with no lysine 3), WNK3, KIAA1566 PRKWNK3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9BYP7 |
| Иммуноген | Recombinant Human Serine/threonine-protein kinase WNK3 protein (1018-1181AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IF, Recommended dilution: IF:1:50-1:200 |
| Примечание | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively (PubMed:16275913, PubMed:16275911, PubMed:16357011). Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed:18768590). Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Serine/threonine-protein kinase WNK3 |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescence staining of 293 cells with CSB-PA861176LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: WNK3 Antibody.