XRCC6/XRCC5 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase II, 80 kDa subunit, CTC box binding factor 85 kDa subunit, CTC85 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P12956/P13010 | | Иммуноген | Synthesized peptide derived from human Ku70/80. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.<br/><br/>Shankar Mitra, AAPG Bulletin, Oct 2006, 90: 1565 - 1584. <br/>Ward Giffin, Mol. Cell. Biol., Jun 1999, 19: 4065 - 4078. <br/>Huichen Wang, Nucleic Acids Res., Sep 2003, 31: 5377 - 5388. <br/>Boris Kysela, J. Biol. Chem., Jun 2003, 278: 22466 - 22474. <br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ku70/80 antibody.
 | Immunofluorescence analysis of A549 cells, using Ku70/80 antibody.
 | Western blot analysis of extracts from LOVO cells, using Ku70/80 antibody.
 | Western blot analysis of extracts from JK cells (Lane 2), using Ku80 antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: XRCC6/XRCC5 Antibody.