ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Антитела / Рекомбинантные антитела Cusabio

TSPO Antibody

Спецификация

Объем50 мкл
СинонимыTranslocator protein, Mitochondrial benzodiazepine receptor, PKBS, Peripheral-type benzodiazepine receptor, PBR, TSPO, BZRP, MBR
Тип антителRecombinant Antibody
SpeciesHuman
UniProt IDP30536
ИммуногенA synthesized peptide derived from human TSPO
Видовая специфичностьHuman, Mouse
ПрименениеELISA, WB, IHC, IF, FC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
КлональностьMonoclonal
ИзотипRabbit IgG
КоньюгатNon-conjugated
БуфферRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очисткиAffinity-chromatography
Области исследованийCardiovascular
АббревиатураTranslocator protein
ПримечаниеCan bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:24814875), but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral-type benzodiazepine receptor, can also bind isoquinoline carboxamides (PubMed:1847678).
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue
All lanes: PBR antibody at 1.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 19, 11 KDa
Observed band size: 19 KDa

IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of PC3 cells with CSB-RA025168A0HU at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating PTGS2 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA025168A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA025168A0HU (3µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)

  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкл 
Кат. номер:CSB-RA025168A0HU
Цена (с НДС 20%):по запросуВ корзину
TSPO AntibodyНаименование: TSPO Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43