| Объем | 50 мкг |
| Синонимы | Thymidylate synthase (TS) (TSase) (EC 2.1.1.45), TYMS, TS |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P04818 |
| Иммуноген | Recombinant Human Thymidylate synthase protein (2-54AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, IP, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Thymidylate synthase |
| Области исследований | Epigenetics and Nuclear Signaling, Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate
All lanes: TYMS antibody at 2.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 32, 27 kDa
Observed band size: 36 kDa
 |
Immunofluorescent analysis of Hela cells using CSB-PA025393LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
 |
Immunoprecipitating TYMS in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA025393LA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA025393LA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
 |
| | |