| Объем | 50 мкл |
| Синонимы | Sulfotransferase family cytosolic 2B member 1 (ST2B1) (Sulfotransferase 2B1) (EC 2.8.2.2) (Alcohol sulfotransferase) (Hydroxysteroid sulfotransferase 2), SULT2B1, HSST2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O00204 |
| Иммуноген | Recombinant Human Sulfotransferase family cytosolic 2B member 1 protein (1-365AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Cancer, Cardiovascular, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Sulfotransferase family cytosolic 2B member 1 antibody at 4µg/ml + MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 42, 40 kDa
Observed band size: 42 kDa
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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA022943ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA022943ESR2HU at dilution of 1:100
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Immunoprecipitating SULT2B1 in MCF-7 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA022943ESR2HU in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA022943ESR2HU (4µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (20µg)
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Наименование: SULT2B1 Antibody.