| Объем | 50 мкл |
| Синонимы | Nucleolysin TIA-1 isoform p40 (RNA-binding protein TIA-1) (T-cell-restricted intracellular antigen-1) (TIA-1) (p40-TIA-1), TIA1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P31483 |
| Иммуноген | Recombinant Human Nucleolysin TIA-1 isoform p40 protein (1-193AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Nucleolysin TIA-1 isoform p40 antibody at 2µg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43, 42, 25 kDa
Observed band size: 43 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA023527DSR1HU at dilution of 1: 100
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Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA023527DSR1HU at dilution of 1: 100
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Immunoprecipitating TIA1 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA023527DSR1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA023527DSR1HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: TIA1 Antibody.