| Объем | 50 мкг |
| Синонимы | Superoxide dismutase [Cu-Zn] (EC 1.15.1.1) (Superoxide dismutase 1) (hSod1), SOD1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P00441 |
| Иммуноген | Recombinant Human Superoxide dismutase [Cu-Zn] protein (2-154AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Destroys radicals which are normally produced within the cells and which are toxic to biological systems. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Superoxide dismutase [Cu-Zn] protein |
| Области исследований | Cancer, Cell biology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: SOD1 antibody at 6µg/ml
Lane 1: Mouse liver tissue
Lane 2: Mouse brain tissue
Lane 3: Hela whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
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Immunofluorescent analysis of 293T cells using CSB-PA02864A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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IHC image of CSB-PA02864A0Rb diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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