SESN1 AntibodyСпецификация| Объем | 50 мкг | | Синонимы | Sestrin-1 (EC 1.11.1.15) (p53-regulated protein PA26), SESN1, PA26 SEST1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q9Y6P5 | | Иммуноген | Recombinant Human Sestrin-1 protein (224-337AA) | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 | | Примечание | Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:25263562, PubMed:26449471). This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May have an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (By similarity). | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | Sestrin-1 | | Области исследований | Cell biology, Metabolism | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate
All lanes: SESN1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57, 64, 50 kDa
Observed band size: 57 kDa
 | Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA021095LA01HU at dilution of 1:100
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Наименование: SESN1 Antibody.