TK1 (Ab-13) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | EC 2.7.1.21, KITH, TK-1, TK1, Thymidine kinase | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P04183 | | Иммуноген | Synthesized non-phosphopeptide derived from human TK around the phosphorylation site of serine 13 (P-G-S(p)-P-S). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP) by catalyzing the phosphotransfer of phosphate from ATP to deoxythymidine (dT) and thymidine (T) in the cell.TK1 expression and activity is regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division. Stability, but not activity, may be regulated via phosphorylation of TK1 at Ser13 by Cdc2 and/or Cdk2, but the precise mode of regulation remains elusive. These observations indicate that TK1 might be a useful marker of cell proliferation, however, recent studies have shown that TK1 plays a more significant role in the DNA damage response.<br/><br/>N Singh, Mol. Cell. Biol., May 1991, 11: 2362 - 2374. <br/>Bin Tian, Am. J. Pathol., Aug 2003, 163: 789. <br/>Ren篓 Gerolami, Cancer Res., Feb 2000, 60: 993 - 1001. <br/>Catia Traversari, Blood, Jun 2007, 109: 4708 - 4715<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from COLO205 cells, using TK (Ab-13) antibody.
 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TK (Ab-13) antibody.
 | Immunofluorescence analysis of HepG2 cells, using TK (Ab-13) antibody.
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Наименование: TK1 (Ab-13) Antibody.