| Объем | 100 мкл |
| Синонимы | Cell cycle checkpoint protein RAD1 (hRAD1) (EC 3.1.11.2) (DNA repair exonuclease rad1 homolog) (Rad1-like DNA damage checkpoint protein), RAD1, REC1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O60671 |
| Иммуноген | Recombinant Human Cell cycle checkpoint protein RAD1 protein (1-282AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200 |
| Примечание | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Cell cycle checkpoint protein RAD1 antibody at 6µg/ml + NIH/3T3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 32, 28, 8 kDa
Observed band size: 32 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA019252ESR1HU at dilution of 1:100
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Наименование: RAD1 Antibody.