| Объем | 50 мкг |
| Синонимы | Melanoma antigen preferentially expressed in tumors (Opa-interacting protein 4) (OIP-4) (Preferentially expressed antigen of melanoma), PRAME, MAPE OIP4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P78395 |
| Иммуноген | Recombinant Human Melanoma antigen preferentially expressed in tumors protein (1-509AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
| Примечание | Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Tags & Cell Markers |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: A375 whole cell lysate, Mouse brain tissue
All lanes: PRAME antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA018603LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA018603LA01HU at dilution of 1:100
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Immunofluorescence staining of HepG2 cells with CSB-PA018603LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: PRAME Antibody.