RPS6KB1 (Ab-424) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | KS6B1, P70-S6K, RPS6KB1, S6K, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P23443 | | Иммуноген | Peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | <p>RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites, rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites . <br /><br />Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851 <br />Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84. <br />Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c <br />Le, X.F, et al. (2003) Oncogene 22: 484</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cell biology,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 293 cells using p70 S6 Kinase(Ab-424) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Ab-424) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using p70 S6 Kinase(Ab-424) Antibody.
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Наименование: RPS6KB1 (Ab-424) Antibody.