| Объем | 100 мкл |
| Синонимы | p65, NFKB3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q04206 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 276(R-P-S(p)-D-R) derived from Human NFκB-p65. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser276 |
| Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Cardiovascular,Cell biology,Metabolism,Microbiology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescence staining of methanol-fixed Hela cells using NFκB-p65 (Phospho-Ser276) Antibody.
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Western blot analysis of extract from Hela cells using NF-κB p65 (phospho-Ser276) antibody. Lane 1: The antibody is not preincubated with blocking peptides. Lane 2: The antibody is preincubated with non- phospho peptide blocking peptides. Lane 3: The antibody is preincubated with phospho peptide blocking peptides.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) Antibody (left) or the same antibody preincubated with blocking peptide (right).
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Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody.
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Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody.
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Наименование: Phospho-RELA (Ser276) Antibody.