Phospho-NFKBIA (Ser32/Ser36) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | I-kappa-B-alpha, IKBA, NF-kappaB inhibitor alpha, NFKBI, NFKBIA | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P25963 | | Иммуноген | Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 | | Примечание | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. <br/><br/>Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823.<br/>Honda, R. et al. (1997) FEBS Lett. 420, 25-27.<br/>Hirao, A. et al. (2000) Science 287, 1824-1827.<br/>Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157.<br/>Chehab,N.H.et al. (1999) Proc .Natl .Acad. Sci. USA 96, 13777-13782.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Ser32/Ser36 | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Immunology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from Hela cells untreated or treated with TNF using IkB-a(Phospho-Ser32/Ser36) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(Phospho-Ser32/Ser36) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using IkB-a(Phospho-Ser32/Ser36) Antibody.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA093630 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-NFKBIA (Ser32/Ser36) Antibody.
Примечание: дополнительная информация (на английском языке). |
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