Phospho-MAPK8/MAPK9/MAPK10 (Thr183/Tyr185) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, JNK-46 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P45983/P45984/P53779 | | Иммуноген | Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V ) derived from Human JNK1/JNK2/JNK3. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 | | Примечание | <p>Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: a-1 and a-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 a-2, and JUND binds only weakly to it. /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity <br /><br />Davis, R.J. (1999) Biochem Soc Symp 64, 1-12. <br />Ichijo, H. (1999) Oncogene 18, 6087-93. <br />Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Thr183/Tyr185 | | Области исследований | Cancer,Immunology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from C6 cells untreated or treated with anisomycin using JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody.
 | Immunofluorescence staining of methanol-fixed Hela cells using JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody.
 | Western blot analysis of extracts from 293 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using JNK1/JNK2/JNK3 (phospho-Thr183/Tyr185) Antibody.
 | | | |
Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA068484 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-MAPK8/MAPK9/MAPK10 (Thr183/Tyr185) Antibody.
Примечание: дополнительная информация (на английском языке). |
|
