| Объем | 100 мкл |
| Синонимы | Vinculin, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P18206 |
| Иммуноген | Peptide sequence around phosphorylation site of Tyrosine 822 (S-G-Y(p)-R-I) derived from Human Vinculin. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.<br /><br />Subauste MC, et al. (2004) J Cell Biol 165, 371-81 <br />Olsen JV, et al. (2010) Sci Signal 3, ra3 <br />J rgensen C, et al. (2009) Science 326, 1502-9</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr822 |
| Области исследований | Isotype/Loading Controls,Cardiovascular,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HeLa cells treated with Forskolin using Phospho-Vinculin (Tyr822) antibody.The lane on the right is treated with the antigen-specific peptide.
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Наименование: Phospho-VCL (Tyr822) Antibody.