| Объем | 100 мкл |
| Синонимы | ACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, kinase ACK1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q07912 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. <br/><br/>Manser E., Nature 363:364-367(1993).<br />Ota T., Nat. Genet. 36:40-45(2004).<br />Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999)<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr284 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HepG2 cells treated with EGF using ACK1 (Phospho-Tyr284) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (Phospho-Tyr284) Antibody.
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Наименование: Phospho-TNK2 (Tyr284) Antibody.