| Объем | 100 мкг |
| Синонимы | Ras-related protein Rab-5A, RAB5A, RAB5 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P20339 |
| Иммуноген | Recombinant Human Ras-related protein Rab-5A protein (1-215AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Ras-related protein Rab-5A |
| Области исследований | Neuroscience, Tags & Cell Markers, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse kidney tissue, Mouse liver tissue, Rat lung tissue
All lanes: RAB5A antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 24, 23 kDa
Observed band size: 24 kDa
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Immunofluorescent analysis of Hela cells using CSB-PA019213EA01HU at dilution of 1: 100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: HL60 whole cell lysate
All lanes: RAB5A antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 24, 23 kDa
Observed band size: 24 kDa
 |
Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA019213EA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA019213EA01HU at dilution of 1:100
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Наименование: RAB5A Antibody.