| Объем | 100 мкл |
| Синонимы | CFL, CFL1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P23528 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.<br/><br/>Kobayashi M, et al. (2006) EMBO J 25(4): 713-26.<br/>Wang Y, et al. (2005) Biol Chem 280(13): 12683-9.<br/>Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68. <br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser3 |
| Области исследований | Neuroscience,Cancer,Cardiovascular,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody.
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Наименование: Phospho-CFL1 (Ser3) Antibody.