| Объем | 100 мкл |
| Синонимы | Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13, TP53, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P04637 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 315 (S-S-S(p)-P-Q) derived from Human p53. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.<br/><br/>Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.<br/>Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539.<br/>Posp |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser315 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells using p53(Phospho-Ser315) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53(Phospho-Ser315) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser315) Antibody.
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Наименование: Phospho-TP53 (Ser315) Antibody.