| Объем | 100 мкл |
| Синонимы | BRCC2, BRCA2, subunit 2, BRCA1/BRCA2-containing complex, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P51587 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 3291 (F-V-S(p)-P-A) derived from Human BRCA2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. <br/><br/>Wooster R., Nature 378:789-792(1995).<br />Tavtigian S.V., Nat. Genet. 12:333-337(1996).<br />Dunham A., Nature 428:522-528(2004).<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser3291 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells using BRCA2 (Phospho-Ser3291) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Наименование: Phospho-BRCA2 (Ser3291) Antibody.