| Объем | 100 мкл |
| Синонимы | SM1A, SMC1A, SMC1L1, SMC1 alpha protein, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14683 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 957 (G-S-S(p)-Q-G) derived from Human SMC1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. <br/><br/>Yang XM. et al. (2004) Mol Cell Biol. 24(12): 5290-5303.<br/>Kim ST. et al. (2002) Genes Dev. 16(5): 560-570.<br/>Revenkova E.et al. (2001) Mol Cell Biol. 21(20): 6984-6998<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser957 |
| Области исследований | Epigenetics and Nuclear Signaling,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells untreated or treated with UV using SMC1(Phospho-Ser957) Antibody.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SMC1(Phospho-Ser957) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-SMC1A (Ser957) Antibody.