| Объем | 100 мкл |
| Синонимы | LDS3, LDS1C, MADH3, JV15-2, HSPC193 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P84022 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 208(N-L-S(p)-P-N) derived from Human Smad3. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser208 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Metabolism,Signal transduction,Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from JK cells using Smad3 (Phospho-Ser208) Antibody.The lane on the left is treated with the antigen-specific peptide.
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Наименование: Phospho-SMAD3 (Ser208) Antibody.