| Объем | 100 мкл |
| Синонимы | ataxia telangiectasia and Rad3-related protein, EC 2.7.11.1, FRAP-related protein, FRP1, kinase ATR, protein kinase ATR |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13535 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 428 (G-I-S(p)-P-K) derived from Human ATR. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC,IHC:1:50-1:100 |
| Примечание | Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser428 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ATR (Phospho-Ser428) antibody. The picture on the right is treated with the synthesized peptide.
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Наименование: Phospho-ATR (Ser428) Antibody.