Phospho-RASGRF1 (Ser916) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | CDC25, GNRP, GRF1, RASGRF1, | | Клональность | Polyclonal Antibody | | Организм | Mouse | | uniprot | Q13972 | | Иммуноген | Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1. | | Источник | Rabbit | | Видовая специфичность | Mouse, Rat | | Применение | ELISA, IHC,IHC:1:50-1:100 | | Примечание | <p>Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.<br/><br/>Huibin Yang, J. Biol. Chem., Apr 2003, 278: 13278 - 13285. <br />Huibin Yang, Mol. Biol. Cell, May 2006, 17: 2177 - 2189. <br />Raymond R. Mattingly, J. Biol. Chem., Dec 1999, 274: 37379. <br />Tie-Nian Zhu, J. Biol. Chem., May 2007, 282: 14816 - 14826.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Ser916 | | Области исследований | Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA256747 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-RASGRF1 (Ser916) Antibody.
Примечание: дополнительная информация (на английском языке). |
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