| Объем | 100 мкл |
| Синонимы | CMT2F, HMN2B, HSP27, HSP28, Hsp25 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P04792 |
| Иммуноген | Peptide sequence around phosphorylation site of serine15 (G-P-S(p)-W-D) derived from Human HSP27. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Involved in stress resistance and actin organization. <br /><br />Tremolada L, et al. (2005) Proteomics. 5(3): 788-795. <br />McGregor E, et al. (2004) Mol Cell Proteomics. 3(2): 115-124. <br />Benn SC, et al. (2002) Neuron. 36(1): 45-56. <br />MacDonald JA, et al. (2002) Mol Cell Proteomics. 1(4): 314-322.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser15 |
| Области исследований | Cancer,Cardiovascular,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated or treated with UV using HSP27(Phospho-Ser15) Antibody.
 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Phospho-Ser15) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 |
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using HSP27(Phospho-Ser15) Antibody.
 |
Western blot analysis of extracts from Hela cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using HSP27 (Phospho-Ser15) Antibody.
 |
| | |
Наименование: Phospho-HSPB1 (Ser15) Antibody.