| Объем | 100 мкл |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P23588 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.<br/><br/>Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.<br/>Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.<br/>Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser422 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated or treated with EGF using eIF4B(phospho-Ser422) Antibody.
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Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B(Phospho-Ser422) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-EIF4B (Ser422) Antibody.