| Объем | 50 мкл |
| Синонимы | Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | P05198 |
| Иммуноген | A synthesized peptide derived from human Phospho-EIF2S1 (S51) |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Epigenetics and Nuclear Signaling |
| Аббревиатура | Eukaryotic translation initiation factor 2 subunit 1 |
| Примечание | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
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Western Blot
Positive WB detected in:Hela whole cell lysate(treated with Calyculin A or EGF)
All lanes:Phospho-EIF2S1 antibody at 1.48µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
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IHC image of CSB-RA007523A51phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-RA007523A51phHU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of A549 cells(treated with 100ng/ml EGF for 20min) with CSB-RA007523A51phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Наименование: Phospho-EIF2S1 (S51) Antibody.